Hotspot

rpoB-trnC-GCA

LSC · 26861-27954 · 1094 bp

Back to Calamus

Pi 0.00408

MarkerSeek score 61.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1094
variable_sites: 11
pi: 0.00408
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1242-1247 bp	1.0	75.1
rpoB-trnC-GCA_p2	TCCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	57.5	1162-1167 bp	1.0	73.3
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	TTTGTTGATCAGGCGACACC	50.0	50.0	58.2	58.8	1279-1284 bp	1.0	73.2
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATTCCGG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	57.5	1163-1168 bp	1.0	73.1
rpoB-trnC-GCA_p5	AATTCTTCCGTCAAGCCCTG	GACACCCGGATTTGAACTGG	50.0	55.0	58.2	58.8	1265-1270 bp	1.0	72.6