Hotspot

atpF-atpH

LSC · 13022-13529 · 508 bp

Back to Calamus

Pi 0.00892

MarkerSeek score 62.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 508
variable_sites: 10
pi: 0.00892
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.9	58.0	603-636 bp	1.0	84.0
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GCTTTTGTTTGCGAATCCCT	50.0	45.0	57.9	57.6	577-610 bp	1.0	80.9
atpF-atpH_p3	CAGTGACCCAAGGAAAGGAA	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.3	58.0	575-608 bp	1.0	79.8
atpF-atpH_p4	CAGTGACCCAAGGAAAGGAAA	ATTTATGGACTGGTCGTGGC	47.6	50.0	58.1	58.0	575-608 bp	1.0	76.6
atpF-atpH_p5	CAGTGACCCAAGGAAAGGAA	GCTTTTGTTTGCGAATCCCT	50.0	45.0	57.3	57.6	549-582 bp	1.0	76.6