Hotspot

rpoC2

LSC · 24897-29339 · 4443 bp

Back to Calamagrostis

Pi 0.00195
MarkerSeek score 38.00
Primer available yes
Label rpoC2

Feature Metadata

label_name
rpoC2
feature_type
gene
region
LSC
length_bp
4443
variable_sites
16
pi
0.00195
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
38.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2_p1 CCCTTAGGCGTTATTGTACCT TGTCCAAGGTGAATTATTGCC 47.6 42.9 57.2 56.5 272 bp 1.0 46.4
rpoC2_p2 CCCTTAGGCGTTATTGTACCT TTGTCCAAGGTGAATTATTGCC 47.6 40.9 57.2 57.2 273 bp 1.0 44.1
rpoC2_p3 TCAAGAAATTTTTGGGCCCT TGTCCAAGGTGAATTATTGCC 40.0 42.9 55.7 56.5 288 bp 1.0 42.5
rpoC2_p4 CCCTTAGGCGTTATTGTACC TGTCCAAGGTGAATTATTGCC 50.0 42.9 55.6 56.5 272 bp 1.0 42.1
rpoC2_p5 TCAAGAAATTTTTGGGCCCTT TGTCCAAGGTGAATTATTGCC 38.1 42.9 56.5 56.5 288 bp 1.0 41.1