Hotspot

ndhH

SSC · 114069-115250 · 1182 bp

Back to Calamagrostis

Pi 0.00183

MarkerSeek score 57.60

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 4
pi: 0.00183
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AGGAGGGTCAAAATGGGTAGA	TGCATTTTGATGAGCCTCCT	47.6	45.0	58.1	57.5	1369 bp	1.0	67.5
ndhH_p2	AAAGGAGGGTCAAAATGGGT	TGCATTTTGATGAGCCTCCT	45.0	45.0	56.9	57.5	1371 bp	1.0	67.3
ndhH_p3	TCCCAAAAGGAGGGTCAAAA	TGCATTTTGATGAGCCTCCT	45.0	45.0	56.8	57.5	1376 bp	1.0	66.8
ndhH_p4	AAAAGGAGGGTCAAAATGGGT	TGCATTTTGATGAGCCTCCT	42.9	45.0	57.7	57.5	1372 bp	1.0	65.3
ndhH_p5	GGAGGGTCAAAATGGGTAGA	TGCATTTTGATGAGCCTCCT	50.0	45.0	56.5	57.5	1368 bp	1.0	64.1