Hotspot

atpA

LSC · 34104-35627 · 1524 bp

Back to Calamagrostis

Pi 0.000328
MarkerSeek score 35.80
Primer available yes
Label atpA

Feature Metadata

label_name
atpA
feature_type
gene
region
LSC
length_bp
1524
variable_sites
1
pi
0.000328
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA_p1 TTCCCCTTGCTTCCGAAAAA TCTTTCTCAACGATTCCTCTTCT 45.0 39.1 57.9 57.3 1610 bp 1.0 49.6
atpA_p2 TTTCCCCTTGCTTCCGAAAA TCTTTCTCAACGATTCCTCTTCT 45.0 39.1 57.9 57.3 1611 bp 1.0 49.6
atpA_p3 TTTTCCCCTTGCTTCCGAAA TCTTTCTCAACGATTCCTCTTCT 45.0 39.1 57.9 57.3 1612 bp 1.0 49.6
atpA_p4 TTTTTCCCCTTGCTTCCGAA TCTTTCTCAACGATTCCTCTTCT 45.0 39.1 57.9 57.3 1613 bp 1.0 49.6
atpA_p5 TTCCCCTTGCTTCCGAAAAA TCTCAACGATTCCTCTTCTACT 45.0 40.9 57.9 56.1 1606 bp 1.0 47.9