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psbE-petL

LSC · 62349-63659 · 1311 bp

Back to Calamagrostis

Pi 0.00407

MarkerSeek score 38.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1311
variable_sites: 9
pi: 0.00407
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	2006-2009 bp	1.0	80.6
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	1995-1998 bp	1.0	80.3
psbE-petL_p3	TTGAGGGCCTCCTAAAAGGA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	2154-2157 bp	1.0	80.2
psbE-petL_p4	TGACAAATAACCAACCCGCA	TATGGGCAGTAGCGAAGAGA	45.0	50.0	58.0	57.9	2129-2132 bp	1.0	80.1
psbE-petL_p5	TGACAAATAACCAACCCGCA	TGCAGTCACGAATAATCCCG	45.0	50.0	58.0	58.1	1770-1773 bp	1.0	79.9