Hotspot

ndhC-trnV-UAC

LSC · 49733-50646 · 914 bp

Back to Calamagrostis

Pi 0.00261

MarkerSeek score 53.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 914
variable_sites: 4
pi: 0.00261
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCCCTGAAATCCAAAATGCC	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.0	57.9	1003-1028 bp	1.0	81.1
ndhC-trnV-UAC_p2	GCCCTGAAATCCAAAATGCC	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.0	58.3	1004-1029 bp	1.0	79.3
ndhC-trnV-UAC_p3	TCCTTCACTAACTGGGGCTA	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.4	57.9	1026-1051 bp	1.0	76.2
ndhC-trnV-UAC_p4	TGGTCCTTCACTAACTGGGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.6	57.9	1029-1054 bp	1.0	76.2
ndhC-trnV-UAC_p5	CTGGTCCTTCACTAACTGGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.2	57.9	1030-1055 bp	1.0	75.1