Hotspot

psaA-ycf3

LSC · 41123-41768 · 646 bp

Back to Calamagrostis

Pi 0.00232

MarkerSeek score 51.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 646
variable_sites: 3
pi: 0.00232
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGTCTGGCCCATTCTTCAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.1	58.0	807-815 bp	1.0	79.7
psaA-ycf3_p2	GGTCTGGCCCATTCTTCAAA	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.1	58.1	803-811 bp	1.0	79.3
psaA-ycf3_p3	GGTCTGGCCCATTCTTCAAA	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.1	57.7	842-850 bp	1.0	77.6
psaA-ycf3_p4	TGTTCTTGAGAAATGGCCGG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	826-834 bp	1.0	76.5
psaA-ycf3_p5	TTTTACTTCTGGTTCCGGCG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	754-762 bp	1.0	76.4