Hotspot

rps16

LSC · 5336-6919 · 1584 bp

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Pi 0.0394

MarkerSeek score 65.50

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1584
variable_sites: 103
pi: 0.0394
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GGGGGCGGATCCAATTAAAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2582-2898 bp	1.0	85.4
rps16_p2	GGGGGCGGATCCAATTAAAA	AATCCTTCCGTCCCAGATCA	50.0	50.0	58.2	58.1	2574-2890 bp	1.0	85.0
rps16_p3	GGGGGCGGATCCAATTAAAA	ATCCTTCCGTCCCAGATCAA	50.0	50.0	58.2	58.1	2573-2889 bp	1.0	85.0
rps16_p4	GGGGGCGGATCCAATTAAAA	TCCTTCCGTCCCAGATCAAT	50.0	50.0	58.2	58.1	2572-2888 bp	1.0	85.0
rps16_p5	AGGGGTGGGGATAAACAGAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	2711-2773 bp	0.75	72.4