Hotspot

rps15-ycf1

SSC · 130524-131414 · 891 bp

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Pi 0.0856

MarkerSeek score 83.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 891
variable_sites: 128
pi: 0.0856
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCAACGCGTTACATAAGG	TCATTGAACCGGTTCGTCTC	50.0	50.0	58.0	57.9	2059-2183 bp	1.0	88.9
rps15-ycf1_p2	TCCCAACGCGTTACATAAGG	CATTGAACCGGTTCGTCTCT	50.0	50.0	58.0	57.9	2058-2182 bp	1.0	88.9
rps15-ycf1_p3	AACACCATGCATTGATGGGT	TCATTGAACCGGTTCGTCTC	45.0	50.0	58.1	57.9	1922-2046 bp	1.0	88.9
rps15-ycf1_p4	AACACCATGCATTGATGGGT	CATTGAACCGGTTCGTCTCT	45.0	50.0	58.1	57.9	1921-2045 bp	1.0	88.9
rps15-ycf1_p5	TTCCCAATTGTTCTGGTGCA	TCATTGAACCGGTTCGTCTC	45.0	50.0	57.9	57.9	2118-2242 bp	1.0	88.2