Hotspot

psaC-ndhE

SSC · 123343-124278 · 936 bp

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Pi 0.111

MarkerSeek score 89.90

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 936
variable_sites: 183
pi: 0.111
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 89.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AGCTTTACATCCTGGCCAAG	CGTAACAGAAAATCAACGCGT	50.0	42.9	57.9	57.8	1095-1289 bp	1.0	81.6
psaC-ndhE_p2	GGCCCGTACACATTGAGTAC	CGTAACAGAAAATCAACGCGT	55.0	42.9	58.4	57.8	1047-1241 bp	1.0	79.9
psaC-ndhE_p3	GCCCGTACACATTGAGTACA	CGTAACAGAAAATCAACGCGT	50.0	42.9	57.6	57.8	1046-1240 bp	1.0	79.7
psaC-ndhE_p4	AGCTTTACATCCTGGCCAAG	TCGTAACAGAAAATCAACGCG	50.0	42.9	57.9	57.6	1096-1290 bp	1.0	79.5
psaC-ndhE_p5	AGGCCCGTACACATTGAGTA	CGTAACAGAAAATCAACGCGT	50.0	42.9	58.4	57.8	1048-1242 bp	1.0	79.2