Hotspot

ycf1-ndhF

SSC · 115491-115846 · 356 bp

Back to Caladium

Pi 0.0907

MarkerSeek score 77.80

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 356
variable_sites: 55
pi: 0.0907
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GATATCAGCAACAACCGGGT	GGGGCGAATCTCGTCTTATC	50.0	55.0	58.0	57.9	706-1539 bp	1.0	89.3
ycf1-ndhF_p2	GATATCAGCAACAACCGGGT	TTATCGAAATGGCTGGCTCC	50.0	50.0	58.0	58.0	1128-1958 bp	1.0	88.0
ycf1-ndhF_p3	AGGGCCCTCTTATCTCTTCC	TTATCGAAATGGCTGGCTCC	55.0	50.0	57.9	58.0	1188-2018 bp	1.0	87.2
ycf1-ndhF_p4	GGGCCCTCTTATCTCTTCCT	TTATCGAAATGGCTGGCTCC	55.0	50.0	57.9	58.0	1187-2017 bp	1.0	87.2
ycf1-ndhF_p5	GATATCAGCAACAACCGGGT	AGTTTCTTTGCGGGTGAAGT	50.0	45.0	58.0	57.9	747-1580 bp	0.75	76.3