Hotspot

trnL-CAA-ndhB

IRb · 99277-100424 · 1148 bp

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Pi 0.0366

MarkerSeek score 87.50

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 1148
variable_sites: 75
pi: 0.0366
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	1208-1275 bp	1.0	88.3
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	1206-1273 bp	1.0	88.3
trnL-CAA-ndhB_p3	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	1203-1270 bp	1.0	86.8
trnL-CAA-ndhB_p4	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	50.0	50.0	57.7	57.9	1207-1274 bp	1.0	86.2
trnL-CAA-ndhB_p5	TCGCGTGTCTACCATTTCAC	TGCAATTGCTCAGGATACCC	50.0	50.0	58.3	57.9	1205-1272 bp	1.0	86.0