Hotspot

petA-psbJ

LSC · 66853-67951 · 1099 bp

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Pi 0.0184

MarkerSeek score 76.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1099
variable_sites: 34
pi: 0.0184
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCGTTACGTGTTCAAGGTCT	TCCGGATTAGGCTCATCTCT	50.0	50.0	57.9	57.3	1227-1316 bp	1.0	78.6
petA-psbJ_p2	CCGTTACGTGTTCAAGGTCT	CCGGATTAGGCTCATCTCTG	50.0	55.0	57.9	57.3	1226-1315 bp	1.0	78.2
petA-psbJ_p3	CCGTTACGTGTTCAAGGTCT	TTCCGGATTAGGCTCATCTCT	50.0	47.6	57.9	58.0	1228-1317 bp	1.0	75.8
petA-psbJ_p4	CCGTTACGTGTTCAAGGTCT	CCTGTGATCGGTTCAATTGG	50.0	50.0	57.9	56.8	1269-1358 bp	1.0	74.3
petA-psbJ_p5	CCGTTACGTGTTCAAGGTCT	CTGGTATTCCTGTGATCGGT	50.0	50.0	57.9	56.7	1277-1366 bp	1.0	73.7