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rps4-trnT-UGU

LSC · 49019-49546 · 528 bp

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Pi 0.0449

MarkerSeek score 76.00

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 528
variable_sites: 38
pi: 0.0449
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TGTGATCTTTTACCCGAGCG	ACCAATTTCGCCATATCCCC	50.0	50.0	58.0	58.0	1200-2148 bp	1.0	87.2
rps4-trnT-UGU_p2	ATCTTTTACCCGAGCGCAAT	ACCAATTTCGCCATATCCCC	45.0	50.0	58.0	58.0	1196-2144 bp	1.0	87.0
rps4-trnT-UGU_p3	TGTGATCTTTTACCCGAGCG	TCCATACCAAGGCTCAATGC	50.0	50.0	58.0	57.9	1239-2187 bp	1.0	86.7
rps4-trnT-UGU_p4	TGTGATCTTTTACCCGAGCG	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.0	58.1	1208-2156 bp	1.0	86.7
rps4-trnT-UGU_p5	TGTGATCTTTTACCCGAGCG	ACCAAGGCTCAATGCAATCA	50.0	45.0	58.0	58.1	1234-2182 bp	1.0	86.6