Hotspot

trnY-GUA-trnE-UUC

LSC · 33366-33483 · 118 bp

Back to Caladium

Pi 0.0551
MarkerSeek score 70.20
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
118
variable_sites
13
pi
0.0551
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CGAATTTACAGTCCGTCCCC TAGCGGTTCAGGACATCTCT 55.0 50.0 58.4 57.9 222-611 bp 1.0 79.0
trnY-GUA-trnE-UUC_p2 CGAATTTACAGTCCGTCCCC TCTAGCGGTTCAGGACATCT 55.0 50.0 58.4 57.9 224-613 bp 1.0 79.0
trnY-GUA-trnE-UUC_p3 ACGAATTTACAGTCCGTCCC TAGCGGTTCAGGACATCTCT 50.0 50.0 57.6 57.9 223-612 bp 1.0 78.8
trnY-GUA-trnE-UUC_p4 ACGAATTTACAGTCCGTCCC TCTAGCGGTTCAGGACATCT 50.0 50.0 57.6 57.9 225-614 bp 1.0 78.8
trnY-GUA-trnE-UUC_p5 CGAATTTACAGTCCGTCCCC GTCTAGCGGTTCAGGACATC 55.0 55.0 58.4 57.8 225-614 bp 1.0 78.5