Hotspot

rpoB-trnC-GCA

LSC · 28534-29993 · 1460 bp

Back to Caladium

Pi 0.0215

MarkerSeek score 72.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1460
variable_sites: 54
pi: 0.0215
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCTCATTTTCATCCCGGAG	GCGACACCCAGATTTGAACT	55.0	50.0	58.0	58.5	1516-1649 bp	1.0	82.7
rpoB-trnC-GCA_p2	CTCATTTTCATCCCGGAGCA	GCGACACCCAGATTTGAACT	50.0	50.0	58.0	58.5	1514-1647 bp	1.0	82.6
rpoB-trnC-GCA_p3	CCCTCATTTTCATCCCGGAG	TAAAGGATTTGCAGTCCCCC	55.0	50.0	58.0	57.5	1491-1624 bp	1.0	82.4
rpoB-trnC-GCA_p4	CCCTCATTTTCATCCCGGAG	CCTTACCACTTGGCCATGTC	55.0	55.0	58.0	58.5	1470-1603 bp	1.0	82.3
rpoB-trnC-GCA_p5	CTCATTTTCATCCCGGAGCA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	57.5	1489-1622 bp	1.0	82.3