Hotspot

trnS-GCU-trnG-UCC

LSC · 9077-10444 · 1368 bp

Back to Caladium

Pi 0.0563

MarkerSeek score 85.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1368
variable_sites: 142
pi: 0.0563
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GCTTTAGTCCACTCAGCCAT	AGGACGAATCACACTTTTACCA	50.0	40.9	57.6	57.7	1431-2178 bp	1.0	66.9
trnS-GCU-trnG-UCC_p2	GCTTTAGTCCACTCAGCCAT	GGACGAATCACACTTTTACCAC	50.0	45.5	57.6	57.6	1430-2177 bp	1.0	66.4
trnS-GCU-trnG-UCC_p3	AGTCCACTCAGCCATCTCTC	AGGACGAATCACACTTTTACCA	55.0	40.9	58.5	57.7	1426-2173 bp	1.0	66.0
trnS-GCU-trnG-UCC_p4	AGTCCACTCAGCCATCTCTC	GGACGAATCACACTTTTACCAC	55.0	45.5	58.5	57.6	1425-2172 bp	1.0	65.6
trnS-GCU-trnG-UCC_p5	TAGTCCACTCAGCCATCTCT	AGGACGAATCACACTTTTACCA	50.0	40.9	57.2	57.7	1427-2174 bp	1.0	63.5