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ycf3

LSC · 41903-43939 · 2037 bp

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Pi 0.000864
MarkerSeek score 58.60
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
2037
variable_sites
5
pi
0.000864
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 CGAGAGGGCCAAGAAACTAA ACGAGTCCGCTTTTATGTTAGT 50.0 40.9 57.2 57.8 80-2241 bp 1.0 56.9
ycf3_p2 CGAGAGGGCCAAGAAACTAA AAACGAGTCCGCTTTTATGT 50.0 40.0 57.2 55.7 80-2243 bp 1.0 55.9
ycf3_p3 CGAGAGGGCCAAGAAACTAA AACGAGTCCGCTTTTATGTT 50.0 40.0 57.2 55.7 80-2242 bp 1.0 55.9
ycf3_p4 CGAGAGGGCCAAGAAACTAAA ACGAGTCCGCTTTTATGTTAGT 47.6 40.9 57.9 57.8 80-2241 bp 1.0 54.6
ycf3_p5 CGAGAGGGCCAAGAAACTAA AAACGAGTCCGCTTTTATGTT 50.0 38.1 57.2 56.5 80-2243 bp 1.0 54.1