Hotspot

ccsA-ndhD

SSC · 114871-115078 · 208 bp

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Pi 0.00558
MarkerSeek score 49.80
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
208
variable_sites
3
pi
0.00558
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TCTGCAATTGTGGCTTCGAT AGCAAGTGACAAGGTCGAAT 45.0 45.0 58.2 57.4 80-372 bp 1.0 76.7
ccsA-ndhD_p2 TTCTGCAATTGTGGCTTCGA AGCAAGTGACAAGGTCGAAT 45.0 45.0 58.4 57.4 80-373 bp 1.0 74.9
ccsA-ndhD_p3 TCTGCAATTGTGGCTTCGAT GCAAGTGACAAGGTCGAATC 45.0 50.0 58.2 57.1 80-371 bp 1.0 74.0
ccsA-ndhD_p4 ATTCTGCAATTGTGGCTTCG AGCAAGTGACAAGGTCGAAT 45.0 45.0 57.4 57.4 80-374 bp 1.0 72.9
ccsA-ndhD_p5 TTCTGCAATTGTGGCTTCGA GCAAGTGACAAGGTCGAATC 45.0 50.0 58.4 57.1 80-372 bp 1.0 72.3