Hotspot

rpl32-trnL-UAG

SSC · 113077-113692 · 616 bp

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Pi 0.00398
MarkerSeek score 51.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
616
variable_sites
9
pi
0.00398
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGAAATCGCTTTCTACAGGT TCCTAAGAGCAGCGTGTCTA 47.6 50.0 58.0 57.9 80-712 bp 1.0 72.4
rpl32-trnL-UAG_p2 GGGAAATCGCTTTCTACAGGT AGCAGCGTGTCTACCAATTT 47.6 45.0 58.0 57.8 80-705 bp 1.0 71.9
rpl32-trnL-UAG_p3 GGGAAATCGCTTTCTACAGGT CCTAAGAGCAGCGTGTCTAC 47.6 55.0 58.0 57.8 80-711 bp 1.0 71.8
rpl32-trnL-UAG_p4 GGGAAATCGCTTTCTACAGGT CTAAGAGCAGCGTGTCTACC 47.6 55.0 58.0 57.8 80-710 bp 1.0 71.8
rpl32-trnL-UAG_p5 GGGAAATCGCTTTCTACAGGT GAGCAGCGTGTCTACCAATT 47.6 50.0 58.0 58.3 80-706 bp 1.0 71.2