Hotspot

trnP-GGG-psaJ

LSC · 66058-66391 · 334 bp

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Pi 0.00431
MarkerSeek score 30.90
Primer available yes
Label trnP-GGG-psaJ

Feature Metadata

label_name
trnP-GGG-psaJ
feature_type
igs
region
LSC
length_bp
334
variable_sites
4
pi
0.00431
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
30.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-GGG-psaJ_p1 ACCCGTGACATTTTGTACCC AAACCCGCTAACGAAACGAA 50.0 45.0 58.1 58.4 80-468 bp 1.0 76.6
trnP-GGG-psaJ_p2 CCCGTGACATTTTGTACCCA AAACCCGCTAACGAAACGAA 50.0 45.0 58.1 58.4 80-467 bp 1.0 76.6
trnP-GGG-psaJ_p3 ACAGGATTTGAACCCGTGAC AAACCCGCTAACGAAACGAA 50.0 45.0 58.1 58.4 80-479 bp 1.0 76.6
trnP-GGG-psaJ_p4 CAGGATTTGAACCCGTGACA AAACCCGCTAACGAAACGAA 50.0 45.0 58.1 58.4 80-478 bp 1.0 76.5
trnP-GGG-psaJ_p5 TGACAGGATTTGAACCCGTG AAACCCGCTAACGAAACGAA 50.0 45.0 58.1 58.4 80-481 bp 1.0 76.5