Hotspot

atpB-rbcL

LSC · 53589-54370 · 782 bp

Back to Cakile

Pi 0.00158
MarkerSeek score 47.90
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
782
variable_sites
5
pi
0.00158
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CATCCAGTACCGGACCAATG CACCAGCTTTGAATCCAACAC 55.0 47.6 58.0 58.0 80-929 bp 1.0 72.1
atpB-rbcL_p2 CATCCAGTACCGGACCAATG ACCAGCTTTGAATCCAACACT 55.0 42.9 58.0 58.1 80-928 bp 1.0 71.8
atpB-rbcL_p3 ATCCAGTACCGGACCAATGA CACCAGCTTTGAATCCAACAC 50.0 47.6 58.1 58.0 80-928 bp 1.0 71.5
atpB-rbcL_p4 TCCAGTACCGGACCAATGAT CACCAGCTTTGAATCCAACAC 50.0 47.6 58.1 58.0 80-927 bp 1.0 71.5
atpB-rbcL_p5 TCCAGTACCGGACCAATGAT ACCAGCTTTGAATCCAACACT 50.0 42.9 58.1 58.1 80-926 bp 1.0 71.2