Hotspot

ndhC-trnV-UAC

LSC · 49759-50641 · 883 bp

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Pi 0.00108
MarkerSeek score 49.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
883
variable_sites
4
pi
0.00108
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCTGGCCCCTTCTTAATTGG TCTACGGTTCGAACCCGTAT 50.0 50.0 57.2 58.2 80-1027 bp 1.0 72.3
ndhC-trnV-UAC_p2 CTGGCCCCTTCTTAATTGGA TCTACGGTTCGAACCCGTAT 50.0 50.0 57.2 58.2 80-1026 bp 1.0 72.2
ndhC-trnV-UAC_p3 TGGCCCCTTCTTAATTGGAG TCTACGGTTCGAACCCGTAT 50.0 50.0 57.2 58.2 80-1025 bp 1.0 72.2
ndhC-trnV-UAC_p4 CTGGCCCCTTCTTAATTGGA TTCGAACCCGTATAGCCCTA 50.0 50.0 57.2 57.6 80-1019 bp 1.0 71.3
ndhC-trnV-UAC_p5 TGGCCCCTTCTTAATTGGAG TTCGAACCCGTATAGCCCTA 50.0 50.0 57.2 57.6 80-1018 bp 1.0 71.3