Hotspot

trnL-UAA-trnF-GAA

LSC · 46729-47414 · 686 bp

Back to Cakile

Pi 0.00613
MarkerSeek score 53.60
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
686
variable_sites
15
pi
0.00613
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.89
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 80-776 bp 1.0 82.1
trnL-UAA-trnF-GAA_p2 AATCGTGAGGGTTCAAGTCC GAACTGGTGACACGAGGATT 50.0 50.0 57.5 57.5 80-773 bp 1.0 78.7
trnL-UAA-trnF-GAA_p3 TTCAAGTCCCTCTATCCCCA TTTGAACTGGTGACACGAGG 50.0 50.0 57.0 58.1 80-765 bp 1.0 78.1
trnL-UAA-trnF-GAA_p4 AATCGTGAGGGTTCAAGTCC TCCTCTGCTCTACCAACTGA 50.0 50.0 57.5 57.4 80-748 bp 1.0 77.8
trnL-UAA-trnF-GAA_p5 AATCGTGAGGGTTCAAGTCC AGTCCTCTGCTCTACCAACT 50.0 50.0 57.5 57.4 80-750 bp 1.0 77.5