Hotspot

psbZ-trnG-UCC

LSC · 35284-35782 · 499 bp

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Pi 0.00249
MarkerSeek score 47.40
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
499
variable_sites
4
pi
0.00249
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 CCGTTGTATTTGCGTCTCCT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.3 57.8 80-689 bp 1.0 77.8
psbZ-trnG-UCC_p2 CTGATGGGTGGTCGAGTAAC CGTCTTCTCCTTGGCAAAGA 55.0 50.0 57.7 57.8 80-671 bp 1.0 77.7
psbZ-trnG-UCC_p3 TGATGGGTGGTCGAGTAACA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.4 57.8 80-670 bp 1.0 77.2
psbZ-trnG-UCC_p4 TATTTGCGTCTCCTGATGGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.4 57.8 80-683 bp 1.0 75.2
psbZ-trnG-UCC_p5 GATGGGTGGTCGAGTAACAA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.2 57.8 80-669 bp 1.0 74.0