Hotspot

trnT-GGU-psbD

LSC · 30901-32048 · 1148 bp

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Pi 0.00228
MarkerSeek score 52.60
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1148
variable_sites
10
pi
0.00228
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG ACCTACAAAAACGAAGCGGT 55.0 45.0 58.1 58.0 80-1322 bp 1.0 80.8
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA ACCTACAAAAACGAAGCGGT 50.0 45.0 58.1 58.0 80-1325 bp 1.0 80.5
trnT-GGU-psbD_p3 ACGGCCCTTTTAACTCAGTG ACCTACAAAAACGAAGCGGT 50.0 45.0 58.1 58.0 80-1339 bp 1.0 80.5
trnT-GGU-psbD_p4 GACGGCCCTTTTAACTCAGT ACCTACAAAAACGAAGCGGT 50.0 45.0 57.8 58.0 80-1340 bp 1.0 79.7
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA ACCTACAAAAACGAAGCGGT 50.0 45.0 58.3 58.0 80-1299 bp 1.0 79.0