Hotspot

rpoB-trnC-GCA

LSC · 25990-27078 · 1089 bp

Back to Cakile

Pi 0.00326
MarkerSeek score 47.90
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1089
variable_sites
15
pi
0.00326
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 GTTCCCTCTTTTCCATCCCC CCGGATTTGAACTGGGGAAA 55.0 50.0 57.9 58.1 80-1178 bp 1.0 79.4
rpoB-trnC-GCA_p2 GTTCCCTCTTTTCCATCCCC AAAAGGATTTGCAGTCCCCC 55.0 50.0 57.9 58.4 80-1160 bp 1.0 77.2
rpoB-trnC-GCA_p3 GTTCCCTCTTTTCCATCCCC TTCAAGTCTCCTGTTGAGGC 55.0 50.0 57.9 57.4 80-1203 bp 1.0 75.6
rpoB-trnC-GCA_p4 TGTTCCCTCTTTTCCATCCC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.1 58.1 80-1179 bp 1.0 73.4
rpoB-trnC-GCA_p5 TCTTTTCCATCCCCGAGCAT CCGGATTTGAACTGGGGAAA 50.0 50.0 59.1 58.1 80-1172 bp 1.0 71.9