Hotspot

trnS-GCU-trnG

LSC · 7754-8323 · 570 bp

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Pi 0.00306
MarkerSeek score 59.10
Primer available yes
Label trnS-GCU-trnG

Feature Metadata

label_name
trnS-GCU-trnG
feature_type
igs
region
LSC
length_bp
570
variable_sites
6
pi
0.00306
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG_p1 CCAACGCTTTAGTCCACTCA AATCAAACCGAGAGACCCTT 50.0 45.0 57.8 56.5 191-780 bp 1.0 68.4
trnS-GCU-trnG_p2 CCAACGCTTTAGTCCACTCA ATCAAACCGAGAGACCCTTT 50.0 45.0 57.8 56.5 191-779 bp 1.0 68.4
trnS-GCU-trnG_p3 AACGCTTTAGTCCACTCAGC AATCAAACCGAGAGACCCTT 50.0 45.0 58.2 56.5 189-778 bp 1.0 68.1
trnS-GCU-trnG_p4 AACGCTTTAGTCCACTCAGC ATCAAACCGAGAGACCCTTT 50.0 45.0 58.2 56.5 189-777 bp 1.0 68.1
trnS-GCU-trnG_p5 CCAACGCTTTAGTCCACTCA AATCAAACCGAGAGACCCTTT 50.0 42.9 57.8 57.2 192-780 bp 1.0 66.4