Hotspot

trnK-UUU-rps16

LSC · 4258-4947 · 690 bp

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Pi 0.00358
MarkerSeek score 59.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
690
variable_sites
7
pi
0.00358
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TAAAAAGGGCGCTCAACCAA 50.0 45.0 58.2 58.3 398-830 bp 1.0 78.1
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TTAAAAAGGGCGCTCAACCA 50.0 45.0 58.2 58.3 399-831 bp 1.0 78.1
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TAAAAAGGGCGCTCAACCAA 50.0 45.0 58.2 58.3 397-829 bp 1.0 78.1
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTAAAAAGGGCGCTCAACCA 50.0 45.0 58.2 58.3 398-830 bp 1.0 78.1
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTAAAAAGGGCGCTCAACC 50.0 50.0 58.2 57.7 399-831 bp 1.0 77.8