Hotspot

trnV-UAC

LSC · 10680-10714 · 35 bp

Back to Cajanus

Pi 0.0286
MarkerSeek score 68.90
Primer available yes
Label trnV-UAC

Feature Metadata

label_name
trnV-UAC
feature_type
tRNA
region
LSC
length_bp
35
variable_sites
1
pi
0.0286
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC_p1 GCAATAAAAATGCACGTTGGG AGTATTAGTAATGAGATAGATTCGA 42.9 28.0 57.3 52.8 164 bp 1.0 40.5
trnV-UAC_p2 GCAATAAAAATGCACGTTGGG AGTATTAGTAATGAGATAGATTCGAT 42.9 26.9 57.3 53.3 164 bp 1.0 40.5
trnV-UAC_p3 TGCACGTTGGGTTTTTAAAC AGTATTAGTAATGAGATAGATTCGA 40.0 28.0 55.6 52.8 154 bp 1.0 40.5
trnV-UAC_p4 AAAAATGCACGTTGGGTTTT AGTATTAGTAATGAGATAGATTCGA 35.0 28.0 55.5 52.8 159 bp 1.0 40.5
trnV-UAC_p5 AAAATGCACGTTGGGTTTTT AGTATTAGTAATGAGATAGATTCGA 35.0 28.0 55.5 52.8 158 bp 1.0 40.5