Hotspot

trnL(tag)-ndhF

SSC · 124821-125767 · 947 bp

Back to Cajanus

Pi 0.0219

MarkerSeek score 75.00

Primer available yes

Label trnL(tag)-ndhF

Feature Metadata

label_name: trnL(tag)-ndhF
feature_type: igs
region: SSC
length_bp: 947
variable_sites: 19
pi: 0.0219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL(tag)-ndhF_p1	TCCTAAGAGCAGCGTGTCTA	CCACATACGGCGAAGATTTT	50.0	45.0	57.9	56.5	1031-1106 bp	1.0	72.5
trnL(tag)-ndhF_p2	AGCAGCGTGTCTACCAATTT	CCACATACGGCGAAGATTTT	45.0	45.0	57.8	56.5	1024-1099 bp	1.0	72.0
trnL(tag)-ndhF_p3	CCTAAGAGCAGCGTGTCTAC	CCACATACGGCGAAGATTTT	55.0	45.0	57.8	56.5	1030-1105 bp	1.0	71.9
trnL(tag)-ndhF_p4	CTAAGAGCAGCGTGTCTACC	CCACATACGGCGAAGATTTT	55.0	45.0	57.8	56.5	1029-1104 bp	1.0	71.9
trnL(tag)-ndhF_p5	AAGATGTCATGCCGCTACTC	CCACATACGGCGAAGATTTT	50.0	45.0	57.8	56.5	1081-1156 bp	1.0	71.6