Hotspot

psaI-ycf4

LSC · 59465-59635 · 171 bp

Back to Cajanus

Pi 0.208

MarkerSeek score 78.70

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 15
pi: 0.208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.42
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GGCAATTGCAATGGCTTCTT	TGCTTATTTTTCGAGACTCCGT	45.0	40.9	57.9	58.1	197-296 bp	1.0	71.4
psaI-ycf4_p2	TTTTCCGGCAATTGCAATGG	TGCTTATTTTTCGAGACTCCGT	45.0	40.9	58.2	58.1	203-302 bp	1.0	70.6
psaI-ycf4_p3	AGTTTTTCCGGCAATTGCAA	TGCTTATTTTTCGAGACTCCGT	40.0	40.9	57.4	58.1	206-305 bp	1.0	67.2
psaI-ycf4_p4	GGCAATTGCAATGGCTTCTT	TGCTTATTTTTCGAGACTCCG	45.0	42.9	57.9	56.4	197-296 bp	1.0	67.0
psaI-ycf4_p5	GGCAATTGCAATGGCTTCTT	GCTTATTTTTCGAGACTCCGT	45.0	42.9	57.9	56.4	196-295 bp	1.0	67.0