Hotspot

accD-psaI

LSC · 58948-59359 · 412 bp

Back to Cajanus

Pi 0.0987

MarkerSeek score 71.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 412
variable_sites: 22
pi: 0.0987
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.54
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCAATCGTACCGCGTTATCT	AAGAAGCCATTGCAATTGCC	45.0	45.0	56.9	57.9	382-566 bp	1.0	77.8
accD-psaI_p2	TCAATCGTACCGCGTTATCT	CCATTGCAATTGCCGGAAAA	45.0	45.0	56.9	58.2	376-560 bp	1.0	77.1
accD-psaI_p3	TCAATCGTACCGCGTTATCTT	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.6	57.9	382-566 bp	1.0	75.5
accD-psaI_p4	TTCAATCGTACCGCGTTATCT	AAGAAGCCATTGCAATTGCC	42.9	45.0	57.6	57.9	383-567 bp	1.0	75.5
accD-psaI_p5	TCAATCGTACCGCGTTATCTT	CCATTGCAATTGCCGGAAAA	42.9	45.0	57.6	58.2	376-560 bp	1.0	74.8