Hotspot

atpA-trnR(tct)

LSC · 51946-52177 · 232 bp

Back to Cajanus

Pi 0.0942

MarkerSeek score 87.20

Primer available yes

Label atpA-trnR(tct)

Feature Metadata

label_name: atpA-trnR(tct)
feature_type: igs
region: LSC
length_bp: 232
variable_sites: 21
pi: 0.0942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 87.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR(tct)_p1	CATTCACTGGGGAAGCAGAA	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	338-346 bp	1.0	71.8
atpA-trnR(tct)_p2	AAGACATTCACTGGGGAAGC	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	342-350 bp	1.0	71.7
atpA-trnR(tct)_p3	GGGGAAGCAGAAGACCTTTT	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.7	57.3	330-338 bp	1.0	71.3
atpA-trnR(tct)_p4	ATTCACTGGGGAAGCAGAAG	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.5	57.3	337-345 bp	1.0	69.5
atpA-trnR(tct)_p5	GACATTCACTGGGGAAGCAG	GGTTCAAATCCTATTGGACGC	55.0	47.6	58.5	57.3	340-348 bp	1.0	69.1