Hotspot

trnT(ggt)-trnE(ttc)

LSC · 30371-31130 · 760 bp

Back to Cajanus

Pi 0.0158

MarkerSeek score 67.80

Primer available yes

Label trnT(ggt)-trnE(ttc)

Feature Metadata

label_name: trnT(ggt)-trnE(ttc)
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 12
pi: 0.0158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT(ggt)-trnE(ttc)_p1	GAACCGATGACTTACGCCTT	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	58.0	58.3	865-870 bp	1.0	79.0
trnT(ggt)-trnE(ttc)_p2	GAACCGATGACTTACGCCTT	TGTCCTGAACCACTAGACGA	50.0	50.0	58.0	57.7	844-849 bp	1.0	78.8
trnT(ggt)-trnE(ttc)_p3	CCATGGCGTTACTCTACCAC	GCTGCCTCCTTGAAAGAGAG	55.0	55.0	58.1	58.3	844-849 bp	1.0	78.5
trnT(ggt)-trnE(ttc)_p4	CCATGGCGTTACTCTACCAC	TGTCCTGAACCACTAGACGA	55.0	50.0	58.1	57.7	823-828 bp	1.0	78.4
trnT(ggt)-trnE(ttc)_p5	TGGCGTTACTCTACCACTGA	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	58.1	58.3	841-846 bp	1.0	78.3