Hotspot

trnL-UAA-trnT(tgt)

LSC · 14320-15435 · 1116 bp

Back to Cajanus

Pi 0.0729

MarkerSeek score 85.00

Primer available yes

Label trnL-UAA-trnT(tgt)

Feature Metadata

label_name: trnL-UAA-trnT(tgt)
feature_type: igs
region: LSC
length_bp: 1116
variable_sites: 59
pi: 0.0729
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnT(tgt)_p1	CTCAGGATTGCCCATTGTGA	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.9	57.9	935-1234 bp	1.0	86.2
trnL-UAA-trnT(tgt)_p2	AGGATTTGGCTCAGGATTGC	GCGATGCTCTAACCTCTGAG	50.0	55.0	58.2	57.9	944-1243 bp	1.0	85.4
trnL-UAA-trnT(tgt)_p3	CCCATTGTGAATTCCAGGGT	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.8	57.9	925-1224 bp	1.0	85.4
trnL-UAA-trnT(tgt)_p4	CTCAGGATTGCCCATTGTGA	GGATTCGAACCGATGACCAT	50.0	50.0	57.9	57.8	967-1266 bp	1.0	85.2
trnL-UAA-trnT(tgt)_p5	TCGGAAAACAGGATTTGGCT	GCGATGCTCTAACCTCTGAG	45.0	55.0	57.7	57.9	953-1252 bp	1.0	84.9