Hotspot

trnV-UAC

LSC · 54936-54974 · 39 bp

Back to Caiophora

Pi 0.0256

MarkerSeek score 64.20

Primer available yes

Label trnV-UAC

Feature Metadata

label_name: trnV-UAC
feature_type: tRNA
region: LSC
length_bp: 39
variable_sites: 1
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC_p1	TGATTGCATGATGGACTCCC	AGAGCTAGGATGTTGAATCATGG	50.0	43.5	57.6	58.0	157 bp	1.0	53.4
trnV-UAC_p2	TGATTGCATGATGGACTCCC	GAGCTAGGATGTTGAATCATGGA	50.0	43.5	57.6	58.0	156 bp	1.0	53.4
trnV-UAC_p3	TGATTGCATGATGGACTCCC	AGCTAGGATGTTGAATCATGGA	50.0	40.9	57.6	56.9	155 bp	1.0	53.3
trnV-UAC_p4	GATTGCATGATGGACTCCCC	AGAGCTAGGATGTTGAATCATGG	55.0	43.5	58.4	58.0	156 bp	1.0	53.2
trnV-UAC_p5	GATTGCATGATGGACTCCCC	GAGCTAGGATGTTGAATCATGGA	55.0	43.5	58.4	58.0	155 bp	1.0	53.2