Hotspot

ndhF

SSC · 113356-115590 · 2235 bp

Back to Caiophora

Pi 0.00492

MarkerSeek score 45.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 11
pi: 0.00492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AACAACGGACGAATTCCACT	ACGCAAGGAAAGAGTAGGAC	45.0	50.0	57.8	57.3	2980-2993 bp	1.0	73.1
ndhF_p2	ACAACGGACGAATTCCACTT	ACGCAAGGAAAGAGTAGGAC	45.0	50.0	57.8	57.3	2979-2992 bp	1.0	73.1
ndhF_p3	ACGGACGAATTCCACTTTCA	ACGCAAGGAAAGAGTAGGAC	45.0	50.0	57.5	57.3	2976-2989 bp	1.0	70.8
ndhF_p4	AACAACGGACGAATTCCACT	CGCAAGGAAAGAGTAGGACT	45.0	50.0	57.8	57.0	2979-2992 bp	1.0	70.8
ndhF_p5	ACAACGGACGAATTCCACTT	CGCAAGGAAAGAGTAGGACT	45.0	50.0	57.8	57.0	2978-2991 bp	1.0	70.8