Hotspot

ndhI-ndhA

SSC · 123238-123319 · 82 bp

Back to Caiophora

Pi 0.0488

MarkerSeek score 77.60

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 82
variable_sites: 4
pi: 0.0488
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	CTTGCGGCTCGTATTGTTTG	GATGGACTTTACCGAGGCTG	50.0	55.0	58.1	58.1	260 bp	1.0	79.8
ndhI-ndhA_p2	AACGATTCGCATGGGATAGG	GATGGACTTTACCGAGGCTG	50.0	55.0	57.8	58.1	307 bp	1.0	79.2
ndhI-ndhA_p3	CTTGCGGCTCGTATTGTTTG	AGATGGACTTTACCGAGGCT	50.0	50.0	58.1	58.1	261 bp	1.0	79.2
ndhI-ndhA_p4	CTTGCGGCTCGTATTGTTTG	TTACCGAGGCTGAGAATGGA	50.0	50.0	58.1	58.1	252 bp	1.0	79.2
ndhI-ndhA_p5	AACGATTCGCATGGGATAGG	AGATGGACTTTACCGAGGCT	50.0	50.0	57.8	58.1	308 bp	1.0	78.6