Hotspot

ndhG-ndhI

SSC · 122366-122733 · 368 bp

Back to Caiophora

Pi 0.0163
MarkerSeek score 50.00
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
368
variable_sites
6
pi
0.0163
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 TGTATTGGTCCAGGTAAATCCA TCGGACAATTTTGAATTCGCC 40.9 42.9 56.8 57.8 431 bp 1.0 45.8
ndhG-ndhI_p2 TGTATTGGTCCAGGTAAATCCA CGGACAATTTTGAATTCGCCT 40.9 42.9 56.8 57.8 430 bp 1.0 45.7
ndhG-ndhI_p3 TGTATTGGTCCAGGTAAATCCA CGGACAATTTTGAATTCGCC 40.9 45.0 56.8 56.3 430 bp 1.0 41.9
ndhG-ndhI_p4 TGTATTGGTCCAGGTAAATCCAT TCGGACAATTTTGAATTCGCC 39.1 42.9 57.3 57.8 431 bp 1.0 41.6
ndhG-ndhI_p5 TGTATTGGTCCAGGTAAATCCAT CGGACAATTTTGAATTCGCCT 39.1 42.9 57.3 57.8 430 bp 1.0 41.6