Hotspot

rpl32-trnL-UAG

SSC · 116890-117783 · 894 bp

Back to Caiophora

Pi 0.0123
MarkerSeek score 58.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
894
variable_sites
11
pi
0.0123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCGCTAAAAGCCTTTTCGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 1002-1004 bp 1.0 80.6
rpl32-trnL-UAG_p2 GCGCTAAAAGCCTTTTCGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.0 58.0 1003-1005 bp 1.0 80.6
rpl32-trnL-UAG_p3 GCGCTAAAAGCCTTTTCGTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.0 57.9 1012-1014 bp 1.0 79.7
rpl32-trnL-UAG_p4 GCGCTAAAAGCCTTTTCGTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.0 57.8 1011-1013 bp 1.0 79.1
rpl32-trnL-UAG_p5 GCGCTAAAAGCCTTTTCGTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.0 57.8 1010-1012 bp 1.0 79.1