Hotspot

ndhF-rpl32

SSC · 115591-116727 · 1137 bp

Back to Caiophora

Pi 0.00992

MarkerSeek score 55.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1137
variable_sites: 11
pi: 0.00992
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TTTGATATAGAAGTGCGTTTTT	50.0	27.3	58.0	52.4	1270-1295 bp	1.0	42.4
ndhF-rpl32_p2	GTTGCCGTCGGAAAAAGAAG	TTTTGATATAGAAGTGCGTTTT	50.0	27.3	58.0	52.4	1271-1296 bp	1.0	42.4
ndhF-rpl32_p3	GTTGCCGTCGGAAAAAGAAG	TTTTTGATATAGAAGTGCGTTT	50.0	27.3	58.0	52.4	1272-1297 bp	1.0	42.4
ndhF-rpl32_p4	TGCCGTCGGAAAAAGAAGAA	TTTGATATAGAAGTGCGTTTTT	45.0	27.3	58.0	52.4	1268-1293 bp	1.0	42.4
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	TTTTGATATAGAAGTGCGTTTT	45.0	27.3	58.0	52.4	1269-1294 bp	1.0	42.4