Hotspot

ycf2-trnL-CAA

IRb · 96407-97105 · 699 bp

Back to Caiophora

Pi 0.00858
MarkerSeek score 63.70
Primer available yes
Label ycf2-trnL-CAA

Feature Metadata

label_name
ycf2-trnL-CAA
feature_type
igs
region
IRb
length_bp
699
variable_sites
6
pi
0.00858
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnL-CAA_p1 ATGGCTTTTCCCGGATGAAA AAATGGTAGACACGCGAGAC 45.0 50.0 57.8 58.0 808 bp 1.0 78.7
ycf2-trnL-CAA_p2 TGGCTTTTCCCGGATGAAAT AAATGGTAGACACGCGAGAC 45.0 50.0 57.8 58.0 807 bp 1.0 78.7
ycf2-trnL-CAA_p3 ATGGCTTTTCCCGGATGAAA TGAAATGGTAGACACGCGAG 45.0 50.0 57.8 58.0 810 bp 1.0 78.7
ycf2-trnL-CAA_p4 TGGCTTTTCCCGGATGAAAT TGAAATGGTAGACACGCGAG 45.0 50.0 57.8 58.0 809 bp 1.0 78.7
ycf2-trnL-CAA_p5 AAGATGGCTTTTCCCGGATG AAATGGTAGACACGCGAGAC 50.0 50.0 58.2 58.0 811 bp 1.0 78.7