Hotspot

petG-trnW-CCA

LSC · 69250-69372 · 123 bp

Back to Caiophora

Pi 0.0244

MarkerSeek score 66.50

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 123
variable_sites: 3
pi: 0.0244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAATACAGACGTGGCGATC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	232 bp	1.0	80.3
petG-trnW-CCA_p2	CAATACAGACGTGGCGATCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	231 bp	1.0	80.3
petG-trnW-CCA_p3	ACAATACAGACGTGGCGATC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.1	58.0	210 bp	1.0	80.2
petG-trnW-CCA_p4	CAATACAGACGTGGCGATCA	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.1	58.0	209 bp	1.0	80.2
petG-trnW-CCA_p5	ACAATACAGACGTGGCGATC	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	196 bp	1.0	79.9