Hotspot

petA-psbJ

LSC · 65765-66797 · 1033 bp

Back to Caiophora

Pi 0.00683

MarkerSeek score 63.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1033
variable_sites: 7
pi: 0.00683
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTTTTGT	GCCGATACTACTGGAAGGAT	45.0	50.0	57.6	56.2	1257-1265 bp	1.0	63.3
petA-psbJ_p2	ACGTGTCCAAGGTCTTTTGT	GCCGATACTACTGGAAGGATT	45.0	47.6	57.6	57.0	1257-1265 bp	1.0	61.4
petA-psbJ_p3	ACGTGTCCAAGGTCTTTTGT	GCCGATACTACTGGAAGGATTC	45.0	50.0	57.6	58.1	1257-1265 bp	1.0	61.0
petA-psbJ_p4	TCCATTACGTGTCCAAGGTC	GCCGATACTACTGGAAGGAT	50.0	50.0	57.2	56.2	1263-1271 bp	1.0	60.6
petA-psbJ_p5	CCATTACGTGTCCAAGGTCT	GCCGATACTACTGGAAGGAT	50.0	50.0	57.2	56.2	1262-1270 bp	1.0	60.5