Hotspot

ndhC-trnV-UAC

LSC · 53223-54323 · 1101 bp

Back to Caiophora

Pi 0.00913

MarkerSeek score 64.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1101
variable_sites: 10
pi: 0.00913
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTCCTTTGCTAATCGGAGCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1240-1246 bp	1.0	76.9
ndhC-trnV-UAC_p2	GTCCTTTGCTAATCGGAGCT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	1239-1245 bp	1.0	74.2
ndhC-trnV-UAC_p3	CTGGTCCTTTGCTAATCGGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.3	57.8	1243-1249 bp	1.0	73.9
ndhC-trnV-UAC_p4	TCTGGTCCTTTGCTAATCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.3	57.8	1244-1250 bp	1.0	73.9
ndhC-trnV-UAC_p5	TGGTCCTTTGCTAATCGGAG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.3	57.8	1242-1248 bp	1.0	73.9