Hotspot

trnF-GAA-ndhJ

LSC · 50855-51542 · 688 bp

Back to Caiophora

Pi 0.0102

MarkerSeek score 65.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 7
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	830-831 bp	1.0	80.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	794-795 bp	1.0	80.0
trnF-GAA-ndhJ_p3	ATGATGCATCGGGAATGGTC	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	850-851 bp	1.0	79.6
trnF-GAA-ndhJ_p4	GATGATGCATCGGGAATGGT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	851-852 bp	1.0	79.5
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	818-819 bp	1.0	79.3