Hotspot

psbC-trnS-UGA

LSC · 37366-37605 · 240 bp

Back to Caiophora

Pi 0.0439

MarkerSeek score 82.30

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 240
variable_sites: 10
pi: 0.0439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	362-364 bp	1.0	83.4
psbC-trnS-UGA_p2	TCTTCGTGGGTCATTTGTGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.1	58.0	445-447 bp	1.0	82.9
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	55.0	50.0	58.2	57.9	342-344 bp	1.0	82.9
psbC-trnS-UGA_p4	TCTTCGTGGGTCATTTGTGG	ATAGCCCCGGTCTTGAAAAC	50.0	50.0	58.1	57.9	425-427 bp	1.0	82.3
psbC-trnS-UGA_p5	TCTTCGTGGGTCATTTGTGG	TCTTTCGGAGAGATGGCTGA	50.0	50.0	58.1	58.1	452-454 bp	1.0	81.8