Hotspot

rpoB-trnC-GCA

LSC · 27822-29009 · 1188 bp

Back to Caiophora

Pi 0.00758

MarkerSeek score 52.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1188
variable_sites: 9
pi: 0.00758
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TTCCTCATTTCCATCCCGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1274 bp	1.0	76.6
rpoB-trnC-GCA_p2	TTTCCTCATTTCCATCCCGG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.6	58.1	1275 bp	1.0	76.4
rpoB-trnC-GCA_p3	TTCCTCATTTCCATCCCGGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	58.4	1256 bp	1.0	74.5
rpoB-trnC-GCA_p4	TTTCCTCATTTCCATCCCGG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	58.4	1257 bp	1.0	74.2
rpoB-trnC-GCA_p5	TTCCTCATTTCCATCCCGGA	CAGCCGAACAGAATAAGGGA	50.0	50.0	58.4	57.3	1323 bp	1.0	71.7